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| Registros recuperados: 56 | |
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| Gueguen, Yannick; Garnier, Julien; Robert, Lorenne; Lefranc, Marie-paule; Mougenot, Isabelle; De Lorgeril, Julien; Janech, Michael; Gross, Paul S.; Warr, Gregory W.; Cuthbertson, Brandon; Barracco, Margherita A.; Bulet, Philippe; Aumelas, André; Yang, Yinshan; Bo, Dong; Xiang, Jianhai; Tassanakajon, Anchalee; Piquemal, Piquemal; Bachere, Evelyne. |
| Antimicrobial peptides play a major role in innate immunity. The penaeidins, initially characterized from the shrimp Litopenaeus vannamei, are a family of antimicrobial peptides that appear to be expressed in all penaeid shrimps. As of recent, a large number of penaeid nucleotide sequences have been identified from a variety of penaeid shrimp species and these sequences currently reside in several databases under unique identifiers with no nomenclatural continuity. To facilitate research in this field and avoid potential confusion due to a diverse number of nomenclatural designations, we have made a systematic effort to collect, analyse, and classify all the penaeidin sequences available in every database. We have identified a common penaeidin signature... |
| Tipo: Text |
Palavras-chave: Nomenclature; Sequence database; Crustacea; Antimicrobial peptide; Penaeid shrimp; Penaeidin. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2332.pdf |
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| Mortensen, Stein Hàkon; Bachere, Evelyne; Le Gall, Ghislaine; Mialhe, Eric. |
| Infectious pancreatic necrosis virus (IPNV), serotype N1 isolated from scallops Pecten maxjmus in Norway, was propagated and used in both inoculation and bath challenge experiments with scallops in vivo. Although virus titers measured in scallop tissues decreased, depuration of virus was not complete during the experimental periods. TPNV was still detectable 11 mo after injection. The highest virus titer was found in the hepatopancreas, but virus was also detectable in other tissues, as well as in the hemolymph. After a bath challenge, uptake of IPNV was shown. Virus was present in hepatopancreas, gonad, kidney, mantle, gill, rectum and in the hemolymph 1 d after the uptake. The titer was highest in the hepatopancreas where virus was detectable at the end... |
| Tipo: Text |
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| Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/1992/publication-2767.pdf |
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| Grizel, Henri; Tige, Gilbert; Gadeau, V.; Bachere, Evelyne. |
| En 1968, un nouveau parasite est mis en évidence par HERRBACB (1971) sur des huîtres plates élevées dans l'Aber Wrach. celui-ci, décrit en 1974 par GRIZEL et coll., sous le nom de Marteilia refringens, s'est propagé progressivement à de nombreux centres d'élevages bretons atteignant le département du Morbihan en 1974 (GRIZEL et coll., 1976). Les premières zones touchées ont été le golfe du Morbihan et la rivière d'Auray, puis en 1975 les rivières de St Philibert et de Crach. La période d'infestation, limitée dans le temps, a lieu durant la période estivale principalement au cours des mois de juillet et août (GRIZEL et coll., 1977). Afin d'enrayer la maladie, et suite aux connaissances acquises, des mesures prophylactiques ont été mises en place, notamment... |
| Tipo: Text |
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| Ano: 1980 |
URL: http://archimer.ifremer.fr/doc/00045/15674/13077.pdf |
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| Bachere, Evelyne; Ifremer, Unité de Pathologie, Immunologie et Génétique Moléculaire des Invertébrés Marins, F-17390 La Tremblade, France; CEV, Centro Experimental de Vilaxoan, Conselleria de Pesca, Villagarcia de Arosa, Spain; CSIC, Consejo Superior Investigaciones Cientificas, Peira de Bouzas S/N, Avda E. Cabello 636208, Vigo, Spain; UCC, University College Cork, Dept of Zoology Cork, Ireland; FCR, Fisheries Research Center, Abbotstown, Castleknock, Dublin 15, Ireland; US, University of Southampton, Dept of Oceanography Southampton S09 5NH, United Kingdom. |
| Bonamia ostreae, an intrahemocytic protozoan parasite infecting the oyster Ostrea edulis, is responsible for an endemic disease affecting all intensive breeding areas of European countries. Epidemiological studies showed variable prevalences, 43% in Galicia (Spain), from 6% to 32% in France and 45% in the most important area of Ireland. The impact of oyster transfers, handlings, high densities have been implicated in the disease development but environmental stressors could be more involved than zootechnical methods. The effect of water temperature, salinity and tidal exposure were particulary examined to assess stress induced changes and determine physiological, biochemical and immunological state indices of oyster populations, which will contribute to... |
| Tipo: Text |
Palavras-chave: Disease; Oyster; Ostrea edulis; Parasitology; Bonamia ostreae; Epidemiology. |
| Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/00273/38383/36713.pdf |
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| Bachere, Evelyne; Chagot, Dominique; Tige, Gilbert; Grizel, Henri. |
| Considering the epizootics which affect the French stock of flat oysters, Ostrea edulis (Grizel, 1985), trials to introduce an Australian oyster, Ostrea angasi, have been undertaken. The spat produced in a hatchery from breeders imported from Australia were reared in the south ofBrittany and in Normandy (Bougrier et al., 1986). Following mass mortalities (65% of individuals) a haplosporidian was observed in this species. Sporogenesis of the parasite was studied using Iight and electron microscopy. |
| Tipo: Text |
Palavras-chave: Haplosporidian; Parasitology; Pathology; Ostrea angasi; Oysters. |
| Ano: 1986 |
URL: http://archimer.ifremer.fr/doc/1986/acte-3112.pdf |
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| Rolland, Jean-luc; Abdelouahab, Mahdia; Dupont, J.; Lefevre, F.; Bachere, Evelyne; Romestand, Bernard. |
| The present study reports the characterization of Ls-Stylicin1, a novel antimicrobial peptide from the penaeid shrimp, Litopenoeus stylirostris. The predicted mature peptide of 82 residues is negatively charged (theoretical pl=5.0) and characterized by a proline-rich N-terminal region and a C-terminal region containing 13 cysteine residues. The recombinant Ls-Stylicin1 has been isolated in both monomeric and dimeric forms. Both display strong antifungal activity against Fusarium oxysporum (1.25 mu M < MIC <2.5 mu M), a pathogenic fungus of shrimp, but lower antimicrobial activity against Gram () bacteria, Vibrio sp. (40 mu M < MIC <80 mu M). However, rLs-Stylicin1 is able to agglutinate Vibrio pennaeicidae in vitro in agreement with its potent... |
| Tipo: Text |
Palavras-chave: Ls-Stylicin 1; Peptide; Shrimp; Antifungal; Agglutination; Vibrio. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00003/11387/8094.pdf |
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| Duperthuy, Marylise; Binesse, Johan; Le Roux, Frederique; Romestand, Bernard; Caro, Audrey; Got, Patrice; Givaudan, Alain; Mazel, Didier; Bachere, Evelyne; Destoumieux Garzon, Delphine. |
| Vibrio splendidus, strain LGP32, is an oyster pathogen associated with the summer mortalities affecting the production of Crassostrea gigas oysters worldwide. Vibrio splendidus LGP32 was shown to resist to up to 10 mu M Cg-Def defensin and Cg-BPI bactericidal permeability increasing protein, two antimicrobial peptides/proteins (AMPs) involved in C. gigas immunity. The resistance to both oyster Cg-Def and Cg-BPI and standard AMPs (polymyxin B, protegrin, human BPI) was dependent on the ompU gene. Indeed, upon ompU inactivation, minimal bactericidal concentrations decreased by up to fourfold. AMP resistance was restored upon ectopic expression of ompU. The susceptibility of bacterial membranes to AMP-induced damages was independent of the ompU-mediated AMP... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00003/11421/8044.pdf |
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| Bachere, Evelyne; Rosa, Rafael Diego; Schmitt, Paulina; Poirier, Aurore; Merou, Nicolas; Charriere, Guillaume; Destoumieux-garzon, Delphine. |
| Oysters are sessile filter feeders that live in close association with abundant and diverse communities of microorganisms that form the oyster microbiota. In such an association, cellular and molecular mechanisms have evolved to maintain oyster homeostasis upon stressful conditions including infection and changing environments. We give here cellular and molecular insights into the Crassostrea gigas antimicrobial defense system with focus on antimicrobial peptides and proteins (AMPs). This review highlights the central role of the hemocytes in the modulation and control of oyster antimicrobial response. As vehicles for AMPs and other antimicrobial effectors, including reactive oxygen species (ROS), and together with epithelia, hemocytes provide the oyster... |
| Tipo: Text |
Palavras-chave: Mollusk; Immunity; Antimicrobial peptide; Hemocyte; Defensin. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00254/36552/35097.pdf |
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| Simon-colin, Christelle; Gueguen, Yannick; Bachere, Evelyne; Kouzayha, Achraf; Saulnier, Denis; Gayet, Nicolas; Guezennec, Jean. |
| Cultured pearls are the product of grafting and rearing of Pinctada margaritifera pearl oysters in their natural environment. Nucleus rejections and oyster mortality appear to result from bacterial infections or from an inappropriate grafting practice. To reduce the impact of bacterial infections, synthetic antibiotics have been applied during the grafting practice. However, the use of such antibiotics presents a number of problems associated with their incomplete biodegradability, limited efficacy in some cases, and an increased risk of selecting for antimicrobial resistant bacteria. We investigated the application of a marine antimicrobial peptide, tachyplesin, which is present in the Japanese horseshoe crab Tachypleus tridentatus, in combination with... |
| Tipo: Text |
Palavras-chave: Pearl oyster; Exopolysaccharide; Tachyplesin; Antimicrobial. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00275/38599/37128.pdf |
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| Duperthuy, Marylise; Schmitt, Paulina; Garzon, Edwin; Caro, Audrey; Rosa, Rafael D.; Le Roux, Frederique; Lautredou-audouy, Nicole; Got, Patrice; Romestand, Bernard; De Lorgeril, Julien; Kieffer-jaquinod, Sylvie; Bachere, Evelyne; Destoumieux-garzon, Delphine. |
| OmpU porins are increasingly recognized as key determinants of pathogenic host Vibrio interactions. Although mechanisms remain incompletely understood, various species, including the human pathogen Vibrio cholera, require OmpU for host colonization and virulence. We have shown previously that OmpU is essential for virulence in the oyster pathogen Vibrio splendidus LGP32. Here, we showed that V. splendidus LGP32 invades the oyster immune cells, the hemocytes, through subversion of host-cell actin cytoskeleton. In this process, OmpU serves as an adhesin/invasin required for beta-integrin recognition and host cell invasion. Furthermore, the major protein of oyster plasma, the extracellular superoxide dismutase Cg-EcSOD, is used as an opsonin mediating the... |
| Tipo: Text |
Palavras-chave: Host-pathogen interaction; Innate immunity; Invertebrate; Mollusk; Oxidative burst. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00031/14177/12166.pdf |
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| De Lorgeril, Julien; Zenagui, Reda; Rosa, Rafael D.; Piquemal, David; Bachere, Evelyne. |
| The cultivated Pacific oyster Crassostrea gigas has suffered for decades large scale summer mortality phenomenon resulting from the interaction between the environment parameters, the oyster physiological and/or genetic status and the presence of pathogenic microorganisms including Vibrio species. To obtain a general picture of the molecular mechanisms implicated in C. gigas immune responsiveness to circumvent Vibrio infections, we have developed the first deep sequencing study of the transcriptome of hemocytes, the immunocompetent cells. Using Digital Gene Expression (DGE), we generated a transcript catalog of up-regulated genes from oysters surviving infection with virulent Vibrio strains (Vibrio splendidus LGP32 and V. aestuarianus LPi 02/41) compared... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00043/15377/12735.pdf |
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| Registros recuperados: 56 | |
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